Buffered Charcoal Yeast Extract (BCYE) | Culture Media

Oct 15, 2013 Buffered Charcoal Yeast Extract Agar for isolation of Buffered Charcoal Yeast Extract Agar (BYCE Agar) is For the isolation, cultivation, and maintenance of Legionella pneumophila and other Legionella species from environmental and clinical specimens. For the presumptive differential identification of Legionella species based on colony color and morphology. Buffered Charcoal Yeast Extract Agar BCYE - Hardy Diagnostics BCYE (Buffered Charcoal Yeast Extract) Selective Agar with GPCV antibiotics (Buffered Charcoal Yeast Extract Agar with Glycine, Vancomycin, Polymyxin, and Cycloheximide) For the selective isolation of Legionella spp. Conforms to ISO 11371 formulation. BUFFERED CYE SELECTIVE AGAR (BCYE w/ GPCV) Remel Buffered CYE Selective Agar w/ GPCV is a solid medium recommended for use in qualitative procedures for selective isolation of Legionella pneumophila from environmental samples.

Culturing, media, and handling of legionella.

Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. It has also been used for the laboratory diagnosis of Acanthamoeba keratitis, Francisella and Nocardia. References Buffered Charcoal Yeast Extract (BCYE) Agar: Composition Buffered charcoal yeast extract agar (BCYE) was developed by Feeley et al and then further modified by Edelstein for selective cultivation of Legionella species. BCYE Agar is an enrichment medium that is used for the isolation and cultivation of Legionella species from environmental samples and …

Francisella colony characteristics - SD Dept. of Health

Yeast extract and peptone (YE + PEP): add 5 g of yeast extract to 10 g of peptone, place in a 500‐ml bottle, add 350 ml of distilled water, and autoclave.. Yeast nitrogen base (10× YNB): 134 g yeast nitrogen base with ammonium sulfate (without amino acids). Dissolve in 1 liter of distilled water and filter sterilize. Nocardiosis Information for Healthcare Workers